Human Ubiquitin
Recombinant Human Ubiquitin (genbank accession number 229532), with a C-terminal extension (HS UBI-SHHHHHH), is expressed in E.coli using our cell growth medium. The protein is purified by non-denaturing methods to homogeneity as determined by SDS-PAGE. Purified protein is lyophilized under conditions preventing deleterious pH changes and packaged under vacuum. Performance is ensured for each batch by nuclear magnetic resonance (NMR) testing.
(Storage: Lyophilized protein in unopened containers may be stored for extended periods at -20°C. Once solubilized, the protein should be stored at 4°C and aseptic conditions should be maintained.)
1H15N-HSQC NMR spectrum of uniformly 15N,13C,2H-labeled Ubiquitin. Sample concentration was 0.7 mM in 50 mM phosphate buffer in 90% H2O/10% D2O at pH=6 with 1mM NaN3. NMR measurements were performed at 30ºC on a 500 MHz system. The spectrum was recorded with 128 msec acquisition time in the proton dimension and 75 msec acquisition time in the nitrogen dimension. All characteristic cross-peaks were observed and chemical shifts are in excellent agreement with published literature data (Wang et al., “Journal of Biomolecular NMR.” 1995, (376-382).
Catalog No. |
Product Name |
Enrichment |
|
17241 |
His-Ubiquitin -13C
|
|
|
17242 |
His-Ubiquitin -15N
|
|
|
17245 |
His-Ubiquitin -13C,15N
|
|
|
17246 |
His-Ubiquitin -13C,2H,15N
|
|
|
17243 |
His-Ubiquitin -2H, 13C
|
|
|
17244 |
His-Ubiquitin -2H, 15N
|
|
|
3223 |
Ubiquitin
|
|
|
C3225 |
Ubiquitin-13C
|
>98% 13C
|
|
C3229 |
Ubiquitin-2H,13C,15N
|
>98%15N, >97%D, >97%13C
|
|
CD3228 |
Ubiquitin-2D, 15N
|
>98%15N, >97% 2D
|
|
CN3227 |
Ubiquitin-13C,15N
|
99%13C, 98%15N
|
|
N3226 |
Ubiquitin-15N
|
98% 15N
|
|
D3224 |
Ubiquitin-2H
|
97% 2H
|
|